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KMID : 0380020030180030197
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 3 p.197 ~ p.201
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Increasing Cell Concentration by the Automatic Addition of Glucose, Ammonium and Phosphate in the Cultivation of a Baker`s Yeast in Alcohol Distillery Wastewater
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Lee Hyeong-Choon
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Abstract
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Automatic addition of glucose, ammonium and phosphate to alcohol distillery wastewater and their control at low concentrations have been carried increase the cell concentration of a baker’s yeast cultivated in the wastewater. Glucose was automatically added using dissolved oxygen as the control parameter, and maintained below 300 mg/L. Ammonium was automatically added by a pH-stat method and maintained in the low range of 12.6~17.4 mM. An automated FIA system, which used an ascorbic acid-based method was developed for the automatic analysis nad addition of phosphate. With this system, the phosphate concentration was succesfully analysed and controlled afrer 19.4 hr in the range 23.3~43.4 mg/L. The cell concentration was increased by 33.0-fold by the addition of these three nutrients. The overall specific growth rate of the yeast was 0.19 .
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KEYWORD
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Baker`s yeast, alcohol distillery wastewater, nutrient addition, automatic analysis of phosphate, flow injection analysis
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